Development of Aphid Resistance Resources in Black Raspberry
- Research Status: Project completed 2020 - 2022
Kelly Vining, Nahla Bassil, & Michael Dossett
Black raspberry is a well-known Pacific Northwest specialty crop with unique flavor and nutritional attributes, but also unique vulnerabilities. A prominent threat facing the crop is the aphid-vectored Black Raspberry Necrosis Virus. Natural genetic resistance to aphids has been identified in black raspberry germplasm in Maine (ME), Michigan (MI), and Ontario (ON), Canada.
In a collaboration between OSU and USDA-ARS researchers, genetic linkage mapping found three separate but linked genetic loci on Rubus Linkage Group 6 (RLG 6). Attempts to target these loci with genetic markers could not clearly distinguish the three from each other. Difficulty in generating markers closely linked to resistance genes was likely due to low overall genetic differentiation among the parents. We continued the work to identify the causal genes conferring aphid resistance using a different approach: gene expression analysis.
Here, we report on the completion of the RNA profiling work to identify genes with changes in expression related to aphid inoculation that differed between resistant and susceptible black raspberry plants.
Project Fact Sheet
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Mulch, C. 2022. Development of Genetic Resources for Rubus Occidentalis from Three Segregating Sources of Resistance to Aphid Amphorophora agathonica. M.S. Thesis, Oregon State University, March 2022.
