Grapevine trunk disease (GTD) is a disease complex that consists of the largest group of fungal pathogens causing disease and progressive vine decline. Due to the complex nature of GTD pathogens, understanding the disease as a phenomenon of single species or multiple species infection is always challenging. In addition, the environmental variables play a greater role in the dominance of one species over another. It is important to understand the species that are predominant in a region and their causal role in GTD development so that targeted disease management programs can be developed. In this research project, we study the diversity of GTD pathogens in grapevines of Northern and Southern Oregon; as well as to compare the different disease management practices including vineyard floor management and pruning wound protection.