Identification of the fungal pathogen causing dry-berry and development of tools for rapid identification

image is not available
Picture of Virginia Stockwell
Virginia Stockwell

William Weldon

image is not available
image is not available
image is not available

Dry-berry, a disease of raspberry and blackberry, whereby immature green berries are killed, was first described in British Columbia, Canada in the 1950’s. The pathogen was named Rhizoctonia rubi, and we have made significant progress in reexamining the identity of the pathogen with molecular methods and determining its fungicide sensitivity.

We characterized DNA sequences of 14 dry-berry fungal isolates and determined that the fungal pathogen causing dry-berry disease is a novel species of Monilinia, not a Rhizoctonia species as previously thought. This has major implications on the types of fungicides that will best manage the disease. Using these DNA sequences, we are now developing PCR-based tools to rapidly confirm dry-berry disease in less than a day. In the longer term, we are preparing high-quality DNA extractions of 4 dry-berry isolates for full genome sequencing, which is ongoing. We also have tested the effectiveness of DMI (FRAC 3), SDHI (FRAC 7), and QoI (FRAC 11) fungicides to limit the growth of the dry-berry pathogen. We also deployed a set of spore traps in 2021 with the goals of obtaining environmental spore samples to determine when dry-berry spores are dispersing in fields and as a resource to test the accuracy of new PCR assays to detect the dry-berry pathogen. Analysis of spore trap data is underway. All together, the information generated with this project may be used to better align the application of the most effective fungicides for dry-berry management during the periods of greatest risk for infection.

View Project Final Report

Weldon, W. A., McGhee, G., Shaffer, B., and Stockwell, V.O. 2021. Optimizing media and temperature conditions for culturing of the dry berry fungal pathogen of Rubus spp. Plant Health 2021, Annual meeting of the American Phytopathological Society. Abstract.

Weldon, W. A., McGhee, G., Shaffer, B., and Stockwell, V.O. Taxonomic reclassification of the fungal pathogen causing dry berry disease of caneberries into the division Ascomycota as Monilinia rubi. Plant Disease 2022.